Fluted pumpkin is a vegetable grown for its succulent leaves, shoots and seeds which are consumed by human and used as forage for livestock feeding. Problems affecting production of fluted pumpkin production include low soil fertility. Therefore, this study examined the influence of fertilizer types on growth, shoot yield and nutritional values of fluted pumpkin at the Teaching and Research Farm, Ladoke Akintola University of Technology, Ogbomoso. The treatments consisted seven fertilizer types (Aleshinloye Grade A, Aleshinloye Grade B, NPK 20:10:10, Poultry manure, Sunshine Grade A, Sunshine Grade B and Urea) applied at 60 kg N/ha, and control. The treatments were arranged in a Completely Randomized Design, replicated four times. Data collected on growth, shoot yield, mineral and proximate analysis of the harvested shoot were subjected to Analysis of Variance (ANOVA) and treatment means separated by Duncan Multiple Range Test (DMRT) at 5% probability level. The results showed that fertilizer types had significant effect (p≤0.05) on number of leaves, nutritional value and shoot yield compared with the control, irrespective of fertilizer types. The optimum growth and shoot yield performance of fluted pumpkin were obtained from NPK20:10:10 fertilizer which was similar to poultry manure and sunshine grade A. The highest nitrogen (0.50%), phosphorus (5.60%), potassium (0.56%), crude protein (3.10 g/100g), and crude fiber (1.80 g/100g) contents were obtained from shoot treated with urea, closely followed by those treated with poultry manure. Control plant had the least nutritional values. In conclusion, NPK20:10:10 fertilizer, poultry manure and sunshine grade A had similar best growth, shoot yield and nutritional values. Therefore, poultry manure could be recommended for cultivation of fluted pumpkin in the study area.